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Viral Detection off Swabs Using the Bead Ruptor Elite in a Novel Two-Step, Direct-to-PCR Technique




Zachary P Morehouse

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19-648 2 mL Reinforced Tubes with Screw Caps & Silicone O-Rings Swab Other 19-042E Bead Ruptor 24 Bead Ruptor Elite Bead Beating RNA RT-qPCR Gel Electrophoresis Coronavirus Virus Bacteria SARS-CoV-2
In the following application, HCoV229E spiked viral swabs were homogenized on the Bead Ruptor Elite to disrupt viral particles. In a direct-to-PCR methodology, homogenate was placed directly onto the PCR instrument for RT-qPCR amplification of HCoV229E nucleocapsid gene followed by PCR product visualization via gel electrophoresis.
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