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Saccharomyces cerevisiae 1-9 mL



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Yeast Cellular DNA
3 mL of an over-night culture of yeast was spun down a resuspended in 480 µL of LD buffer and lyticase solution from the Omni Yeast DNA kit . Sample was incubated at 30° C then 200 µL of Lysis buffer and 10 µL of Antifoam were added. Sample was transferred to a 2 mL tube with 0.1 mm ceramic beads. Sample was processed on the Bead Ruptor 24 at 5 m/s for 2 cycles of 30 seconds with a 45 second dwell.
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