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RNA Extraction from Human Coronavirus with OMNI Universal RNA Purification Kit and Bead Ruptor Elite

Analyte:

RNA

Authors:

Zachary P Morehouse

Created On:

2020

Tags:
26-010V Universal RNA Purification Kit Cell Culture Other 19-042E Bead Ruptor 24 Bead Ruptor Elite Bead Beating RNA RT-qPCR Gel Electrophoresis Coronavirus Mammal SARS-CoV-2
In the following application, Human Coronavirus 229E (HCoV229E) was homogenized using the Bead Ruptor Elite. Using the Omni Universal RNA Purification Kit, RNA was extracted and HCoV229E nucleocapsid gene was amplified by RT-qPCR followed by PCR product visualization via gel electrophoresis.
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