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Detection and Cross Contamination Evaluation of Roundup Ready Soy via Mechanical Shear Homogenization using the GLH-850

Analyte:

DNA

Authors:

Shari Garrett

Created On:

2016

Tags:
30750H G10-95W Hard Tissue Omni Tip Plastic Homogenizing Probes (7 x 110 mm) 10 x 115 mm Saw Tooth (Coarse) Generator Probe Fruit Vegetable Plant 20-010 General Laboratory Homogenizer GLH 850 Rotor Stator DNA DNA Spectrophotometry Gel Electrophoresis PCR Soy Beans
Description Products Used Questions/Comments
In the following application, organic soybeans were homogenized using the GLH-850 fitted with a 10 mm stainless steel probe followed by DNA extraction and quantification via spectrophotometry. DNA was visualized via gel electrophoresis, and amplified via qPCR for the detection of the 5’-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene. This study was performed to quantify cross-contamination during rotor-stator homogenization.
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