OMNI’S DIRECT TO PCR METHOD
FOR SARS-COV-2 DETECTION

The Omni team has developed a novel direct-to-PCR workflow for SARS-COV-2 detection off nasopharyngeal swab samples. Based around efficient and effective viral lysis using the Bead Ruptor Elite Homogenizer, the user can homogenize 24 samples in only 30 seconds and place that lysate directly into an RT-qPCR reaction for virus detection.

OMNI’S DIRECT TO PCR METHOD WILL:

  • Eliminate the traditional nucleic acid extraction step
  • Save you valuable time and money
  • Improved your test sensitivity (> 96%)
  • Achieve 100% specificity when compared to standard workflows
Viral Detection off Swabs Using the Bead Ruptor Elite in a Novel Two-Step, Direct-to-PCR Technique

The data presented in the poster, A Novel Direct-to-PCR Viral Detection Method Developed to Improve SARS-COV-2 Testing Efforts. Received 1st place at the Michigan Osteopathic Association Scientific Research Competition on October 31st, 2020. The data presented in this poster outlines our initial direct-to-PCR validation with HCoV229E, along with SARS-COV-2 sample direct-to-PCR validation. The HCoV229E direct-to-PCR method demonstrates 96.3% sensitivity and the SARS-COV-2 sample direct-to-PCR method demonstrates 96.4% sensitivity.

BEAD RUPTOR ELITE

Bead Mill Homogenizer

Bead Ruptor Elite

High throughput? No problem

  • Process hundreds of samples a day
  • No cool down between runs
  • Wide range of accessories

Complete Direct to PCR SARS-COV-2 Solution
SKU: 19-040COV

Includes:

  • Bead Ruptor Elite Homogenizer
    SKU: 19-040E
  • 2 mL Kit Finger Plate
    SKU: 19-010-310
  • 2 mL Tough Tubes/caps
    SKU: 19-648

Omni's Peer Reviewed Papers

Using human coronavirus 229E as the model system, this workflow was tested and optimized for laboratory implementation. See our recent publication in the Virology Journal outlining this process on in vitro testing.

Using human coronavirus 229E as the model system, this workflow was tested and optimized for laboratory implementation. See our recent publication in the Virology Journal outlining this process on in vitro testing.

Viral Detection off Swabs Using the Bead Ruptor Elite in a Novel Two-Step, Direct-to-PCR Technique

Following the HCoV-229E testing, this methodology was validated using SARS-COV-2 samples. You can see the data and workflow from our SARS-COV-2 testing in the Pre-Print server manuscript listed below.