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SKU 26-011B

Bead Mill Bacterial RNA Purification Kit

Pack Size: 50 Pack

Bead Mill Bacterial RNA Purification Kit


Bead Mill Bacteria RNA Purification Kit - 50 Prep

The Omni Bead Mill Bacteria RNA Purification Kit contains silica-based spin-capture columns, pre-filled bead beating tubes, and nontoxic reagents. The optimized reagents are designed specifically for RNA extraction from bacteria through bead mill homogenization and utilize highly denaturing buffer conditions to inactivate RNase's. After sample lysis using the Omni pre-filled bead tubes, the RNA is purified through spin-column capture in less than 60 minutes. RNA purified using the Omni Bacteria RNA Kit is ready for downstream applications such as RT-PCR. Pre-filled bead tubes are rigorously tested to be free of nucleases.

Product Features:

  • Includes pre-filled bead beating tubes, spin-columns, and nontoxic reagents
  • RNA isolation in <20 minutes after lysis
  • No organic extractions
  • Suitable for up to 3 mL log-phase bacteria cells
  • Includes 2 mL tubes pre-filled with 0.1 mm ceramic beads
  • Certification: Pre-filled bead tubes certified RNase & DNase Free

Illustrated Protocol

Bacterial RNA

Example Results

Total RNA purified using the Omni Bead Mill Bacterial RNA Purification Kit. RNA was extracted from Esherichia coli (E. coli) at 4.4 x 10^8, 2.2 x 10^8, and 1.1 x 10^8 cfu/mL on the Bead Ruptor Elite, in pre-filled 2 mL tubes with 0.1 mm glass beads at 3.1 m/s for 60 sec. Purified RNA was analyzed on the Agilent 2100 Bioanalyzer. Average RNA Integrity Numbers (RIN) was 8.5.

Ordering Information: Unit includes 50 spin-columns, 50 bead beating tubes prefilled with 0.1 mm ceramic beads, reagents suitable for 50 purifications and instruction guide

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