Nucleic Acid Extraction from HepG2 Cells: An Integrated Solution Utilizing the Bead Ruptor Elite Homogenizer, chemagic 360 Nucleic Acid Extractor and JANUS G3 Automated Workstation

Analyte:

DNA

Authors:

Gabriella Ryan

Created On:

2021

Tags:

19-628 Hard Tissue Homogenizing Mix 2.8 mm Ceramic (2 mL Reinforced Tubes) Nuclease Free Liver Animal 19-042E Bead Ruptor 24 Bead Ruptor Elite Bead Beating DNA DNA Spectrophotometry qPCR Gel Electrophoresis Automated Nucleic Acid Extraction

Description Products Used Questions/Comments

In the following application, murine liver tissue was homogenized on the Omni Bead Ruptor Elite and nucleic acid was extracted on the chemagic 360. Nucleic acid was analyzed via spectrophotometry and 18S rRNA gene was amplified via qPCR followed by PCR product visualization on gel electrophoresis.