From Sample Prep to Sonication: A ChIP-Seq Analysis Workflow from Various Tissues Using the OMNI Bead Ruptor Elite™ Bead Mill Homogenizer and PIXUL™ Multi-Sample Sonicator
Traditional methods of tissue homogenization have limitations such as sample loss and low amounts of cells being available, which in turn yields a low amount of starting material that can make some experiments impossible. With such limitations from small amounts of starting material and difficult-to-process samples, consistency in data and consistency in experiments is more important than ever. High quality tissue homogenization and consistent shearing is pivotal to generate data. Herein, we demonstrate a robust workflow for ChIP-Seq sample preparation and analysis from clinically relevant tissue types using the Bead Ruptor Elite™ Bead Mill Homogenizer to dissociate tissues and the PIXUL™ Multi- Sample Sonicator for ChIP-Seq sample preparation.