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Escherichia coli 1-9 mL



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Bacteria Cellular DNA
3 mL of an over-night culture of bacteria was spun down a resuspended in 200 µL of Tris-EDTA and lysozyme buffer. Sample was incubated at 37° C,then 100 µL of Lysis buffer from the Omni Bacterial DNA kit and 10 µL of Antifoam were added. Sample was transferred to a 2 mL tube with 0.1 mm ceramic beads. Sample was processed on the Bead Ruptor 24 at 4.35 m/s for 45 seconds.
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