Saccharomyces cerevisiae 1-9 mL
40 mL culture of yeast was spun down a resuspended in 480 µL of LD buffer and lyticase solution from the Omni Yeast RNA kit . Sample was incubated at 30° C then 350 µL of Lysis buffer and 10 µL of Antifoam were added. Sample was transferred to a 2 mL tube with 0.1 mm ceramic beads. Sample was processed on the Bead Ruptor 24 at 4.2 m/s for 2 cycles of 30 seconds with a 45 second dwell.